tag:blogger.com,1999:blog-7857054149675424609.post3577197442197091015..comments2024-02-24T08:58:08.581+00:00Comments on RNAi Therapeutics: Journal Club: Study Shows TLR3 Induction by siRNAs with Anti-angiogenic Effects, Questioning Ongoing RNAi Clinical Trials for Wet AMDDirk Hausseckerhttp://www.blogger.com/profile/18320439857875629714noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-7857054149675424609.post-34901778790889161862008-03-30T03:30:00.000+00:002008-03-30T03:30:00.000+00:00I don't want to comment on the Galenea studies, bu...I don't want to comment on the Galenea studies, but note that there is a widespread feeling that the flu mouse models may be tricky to work with.<BR/><BR/>With regards to ALN-RSV01 it was reported in the latest study that efficacy wasn't correlated with innate immune activation, although one cannot rule out that the specific cytokines looked at, the timing of the analysis, and taking samples fromDirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-54265513036900403402008-03-29T21:47:00.000+00:002008-03-29T21:47:00.000+00:00It is good point that it is hard to exclude the ro...It is good point that it is hard to exclude the role of interferon response played in the inhibition of viral replication in respiratory epithelial cells. As you know, the publication of RNAi on influenza virus originated for Galenea and later licensed to Nastech showed viral inhibition in mice with delivery vehicle (I assume that naked siRNA wasn't working because they used PEI as delivery Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-1240540766300711352008-03-29T07:09:00.000+00:002008-03-29T07:09:00.000+00:00Excellent question. It appears that some mucosa, ...Excellent question. It appears that some mucosa, such as those of the lung and cervix, for some reason take up unformulated siRNA quite well. Alnylam has also shown that fluorescently labeled siRNAs went into the same cells in the mouse lung where RSV replicates. But in any case, there is a reasonable chance that cytokine activation may play a role in the antiviral activity of ALN-RSV01. Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-21000869770736280862008-03-28T23:04:00.000+00:002008-03-28T23:04:00.000+00:00If this can be generalized, how to understand Alny...If this can be generalized, how to understand Alnylam's RSV program which is using naked sRNA to inhibit virus? Are something still not discovered there for specific viral gene inhibition?Anonymousnoreply@blogger.com