tag:blogger.com,1999:blog-7857054149675424609.post5982812291809431434..comments2024-02-24T08:58:08.581+00:00Comments on RNAi Therapeutics: Brewed up in Seattle: Tekmira and Halo-Bio Seek to Capitalize on Multi-Targeting Potential of RNAi Therapeutics with Multi-Valent RNAi TriggersDirk Hausseckerhttp://www.blogger.com/profile/18320439857875629714noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-7857054149675424609.post-89702749297920226292011-09-11T01:41:22.643+00:002011-09-11T01:41:22.643+00:00MV-RNAi? Um, ok. With a few more of these moves Te...MV-RNAi? Um, ok. With a few more of these moves Tekmira might become the new MDRNA.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-51837013643116612182011-08-29T22:02:40.751+00:002011-08-29T22:02:40.751+00:00OT: when can we start hearing about alny and tkmr ...OT: when can we start hearing about alny and tkmr litigation outcome? this is dragging down TKMR.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-29071884172374417882011-08-29T21:53:53.267+00:002011-08-29T21:53:53.267+00:00I don't think I stated that. Please re-read. I...I don't think I stated that. Please re-read. It is the USPTO that has argued that the 3' overhangs were implicit in T-I.Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-87753492619507547272011-08-29T04:55:00.209+00:002011-08-29T04:55:00.209+00:00I am confused about your statement that part of th...I am confused about your statement that part of the defense of T II is that the 3' overhang was obvious in the T I patent application. I thought that the defense of T II was dependent on the fact that the 3' overhang was not obvious in the T I patent application. Can you clarify?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-80072012317972984182011-08-27T20:01:25.621+00:002011-08-27T20:01:25.621+00:00I understand your question now. The answer is ...I understand your question now. The answer is 'no', since the strand that interacts with the target mRNA will have been unwound and separated from the Mercedes star structure. It is unclear, however, what happens to the other 2 strands once the first one has been removed.Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-72798219550423474332011-08-27T09:37:56.677+00:002011-08-27T09:37:56.677+00:00Thanks for your answer. With regard to 2 I meant: ...Thanks for your answer. With regard to 2 I meant: does the trigger on the spoke of your mercedes benz not have to bind to the host RNA? If so once bound does that not restrict the ability of the other fixed spokes to bind to areas of RNA that may not be in the immediate vicinity. 3 independent strands would not be restricted in this fashion. Not sure if I conveyed clearly enough!Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-62694441996153673562011-08-26T17:15:16.670+00:002011-08-26T17:15:16.670+00:001. No. One of the reasons why T1/2 don't appl...1. No. One of the reasons why T1/2 don't apply (even if they issued in a stronger manner) is the shorter double-stranded region of Halo's mv-RNAs.<br /><br />2.Not sure whether I understand you correctly, but any crowding/competition effect etc should not be much different from the use of 3 separate Tuschl-type siRNAs.Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-18006977434183478622011-08-26T13:18:05.404+00:002011-08-26T13:18:05.404+00:00Interesting development, a couple of questions:
1....Interesting development, a couple of questions:<br />1. Do T1/2 apply to these composite triggers - do the individual fused strands/elements still contain T1/2 attributable features?<br />2. Although fusing three triggers together sounds efficacious, would the fusion of these three triggers together not cause three dimensional geospatial problems with targeting three different regions of the mRNAAnonymousnoreply@blogger.com