tag:blogger.com,1999:blog-7857054149675424609.post8529714406241409088..comments2024-02-24T08:58:08.581+00:00Comments on RNAi Therapeutics: SNALP Works!Dirk Hausseckerhttp://www.blogger.com/profile/18320439857875629714noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-7857054149675424609.post-60473981029945802552011-12-02T14:32:48.490+00:002011-12-02T14:32:48.490+00:00It is the time-course of the knockdown that is ver...It is the time-course of the knockdown that is very typical of a bona fide RNAi knockdown induced by SNALP in the liver. A TTR knockdown by some funny inflammatory pathway (which I have not seen yet that this is an issue for the TTR gene) would a) be much more transient; b) more shallow. <br /><br />You will NEVER see a control siRNA as a control. There are both scientific and ethical reasons Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-13237452824512343742011-12-02T00:34:09.686+00:002011-12-02T00:34:09.686+00:00Thanks for posting this Dirk. Can you please clari...Thanks for posting this Dirk. Can you please clarify; when you say, "There is no doubt that this was an RNAi-mediated response," is this based on the timeframe and degree of TTR knockdown? Or, in addition to this, the lack of liver toxicity as a means to verify that the knockdown is not due to inflammation (I am particularly curious about this because, based solely on some of the cell Nicknoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-3280678625336371942011-11-29T13:29:57.158+00:002011-11-29T13:29:57.158+00:00Interesting!!! "Delivery through" is th...Interesting!!! "Delivery through" is the interesting term. I wonder Greene meant to say that, it almost means liver is a processing plant and converting the complex for effective delivery to other target sites??? Is that what he meant?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-63128297404450761792011-11-29T00:53:22.967+00:002011-11-29T00:53:22.967+00:00I noted the same and was surprised, given their ow...I noted the same and was surprised, given their own delivery activities that go beyond the liver. There are many ways to interpret this. If this becomes the new message, it would make for a good speculative blog topic.Dirk Hausseckerhttps://www.blogger.com/profile/18320439857875629714noreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-51416187474523441022011-11-28T14:12:30.956+00:002011-11-28T14:12:30.956+00:00Dirk
I don't know if you had a chance to hear...Dirk<br /><br />I don't know if you had a chance to hear the podcast of a Barry Greene, Alnylam COO, interview on the Burrill Report. Link: http://www.burrillreport.com/content/podcast/BRP-11-28-11.mp3<br />Greene indicated the focus of Alnylam's pipeline in the future will be delivery through the liver, presumably with LNP, for any targets that can be reached through that process. AnyAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-7857054149675424609.post-68424087746712816022011-11-24T04:23:36.481+00:002011-11-24T04:23:36.481+00:00Thank you for all of the info you provide that enh...Thank you for all of the info you provide that enhances our opportunity to make good RNAI investment decisions!<br /><br />Happy Thanksgiving to you and yours! God Bless!<br /><br />BarryBarrynoreply@blogger.com